Combining the in vivo comet and micronucleus assays: a practical approach to genotoxicity testing and data interpretation.
Mutagenesis
; 25(2): 187-99, 2010 Mar.
Article
em En
| MEDLINE
| ID: mdl-19969526
ABSTRACT
Despite regulatory directives requiring the reduction of animal use in safety testing, recent modifications to genotoxicity testing guidelines now propose the use of two in vivo genotoxicity assays as a follow-up to an in vitro positive (International Conference on Harmonization Consensus Draft Guidance S2[R1] released March, 2008). To address both goals, the in vivo comet and micronucleus (MN) assays can be successfully combined into one informative study. Combining these two assays with such differences in sensitivity, endpoints measured and the type of data generated significantly improves upon the current standard capabilities for detecting genotoxicity without requiring additional animals. But to take full advantage of the benefits of incorporating the comet assay in safety testing, these same differences must be recognized and considered. Developed from over 15 years experience using the in vivo comet and MN assays in genotoxicity testing of chemicals and pharmaceuticals, this paper presents guidelines for the appropriate experimental design, dose selection and data interpretation for combined in vivo comet/MN assay studies. To illustrate the approach, data from combined assay studies are presented and discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Projetos de Pesquisa
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Dano ao DNA
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Testes para Micronúcleos
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Interpretação Estatística de Dados
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Ensaio Cometa
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Testes de Mutagenicidade
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Mutagênicos
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article