[Quantitative assessment of in vitro neutralizing activity of secretory factors from rat lungs against influenza virus].
Vestn Ross Akad Med Nauk
; (11): 46-9, 2009.
Article
em Ru
| MEDLINE
| ID: mdl-20017408
ABSTRACT
Secretory factors were isolated by lung wash followed by centrifugation to remove cells, dialysis of supernatant to remove NaCl salt, lyophilization of the lavage fluid and resuspention of the lyophilization product in an isotonic NaCl solution. It was shown that biological activity of influenza virus /Aichi/2/68 (3N2) significantly decreased (p = 0,01) from 8,17 +/- 0,10 to 7,14 +/- 0,20 IgEID50/ml during its incubation with secretory factors at 37 degrees C for 1 hr and to 7,92 +/- 0,17 IgEID50/ml in isotonic NaCl solution in the absence of these factors. Their concentration in the incubation medium was estimated to be 9.1 +/- 0.7% of their level in the lungs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alvéolos Pulmonares
/
Surfactantes Pulmonares
/
Líquido da Lavagem Broncoalveolar
/
Infecções por Orthomyxoviridae
/
Mucosa Respiratória
/
Vírus da Influenza A Subtipo H3N2
/
Pulmão
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Ru
Ano de publicação:
2009
Tipo de documento:
Article