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Performance of laboratory diagnostics for the detection of influenza A(H1N1)v virus as correlated with the time after symptom onset and viral load.
Cheng, Peter K C; Wong, Kitty K Y; Mak, Gannon C; Wong, Ann H; Ng, Anita Y Y; Chow, Salina Y K; Lam, Ricky K H; Lau, C S; Ng, K C; Lim, Wilina.
Afiliação
  • Cheng PK; Centre for Health Protection, Department of Health, Hong Kong, People's Republic of China.
J Clin Virol ; 47(2): 182-5, 2010 Feb.
Article em En | MEDLINE | ID: mdl-20022296
BACKGROUND: To diagnose influenza A(H1N1)v virus infection, accurate and rapid detection are important. However, there is scanty data on the performance of various laboratory diagnostics. OBJECTIVE: To compare the performance of rapid antigen test (RAT), viral culture and RT-PCR for the detection of influenza A(H1N1)v virus and to correlate their performance with the time after symptom onset and viral load. STUDY DESIGN: From May 1, 2009 to June 25, 2009, respiratory samples were collected from 5740 individuals suspected of having influenza A(H1N1)v infection. The performance of viral culture and RT-PCR were investigated and correlated with the time after symptom onset. The sensitivity of RAT ESPLINE influenza A & B-N (Fujirebio Inc, Tokyo) was evaluated using a subset of 60 samples from patients diagnosed as having influenza A(H1N1)v infection. RESULTS: Using respiratory samples from 587 patients diagnosed with influenza A(H1N1)v infection, comparison of laboratory diagnostics showed viral culture and RT-PCR gave comparable results with overall sensitivity of 93.9% and 98.1%, respectively. For RAT, when testing a subset of 60 samples collected < or =3 days following symptom onset, the sensitivity was 62%. CONCLUSIONS: Although viral shedding is prolonged and of higher titre in influenza A(H1N1)v infection, RAT showed a low sensitivity of 62% among patients presenting < or =3 days after symptom onset. Viral culture showed comparable performance with RT-PCR and with sensitivity better than that documented for seasonal influenza.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carga Viral / Técnicas de Laboratório Clínico / Influenza Humana / Vírus da Influenza A Subtipo H1N1 Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Adolescent / Adult / Aged / Child / Child, preschool / Humans / Infant / Male / Middle aged Idioma: En Ano de publicação: 2010 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carga Viral / Técnicas de Laboratório Clínico / Influenza Humana / Vírus da Influenza A Subtipo H1N1 Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Adolescent / Adult / Aged / Child / Child, preschool / Humans / Infant / Male / Middle aged Idioma: En Ano de publicação: 2010 Tipo de documento: Article