Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria.
Ann Clin Microbiol Antimicrob
; 9: 1, 2010 Jan 12.
Article
em En
| MEDLINE
| ID: mdl-20067633
ABSTRACT
BACKGROUND:
We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.METHODS:
Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.RESULTS:
By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed blaSHV-12 associated to the insertion sequence, IS26.CONCLUSION:
This is the first study demonstrated the occurrence of SHV-12 in Nigeria.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Beta-Lactamases
/
Enterobacter aerogenes
/
Infecções por Enterobacteriaceae
Limite:
Child, preschool
/
Humans
/
Male
País/Região como assunto:
Africa
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article