[Relationship between neuronal restricted silencing factor and induced differentiation from rat mesenchymal stem cells to neurons].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
; 31(6): 702-6, 2009 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-20078938
ABSTRACT
OBJECTIVE:
To analyze the change of the neuronal restricted silencing factor (NRSF) gene as well as the NRSF regulation genes in beta-mercaptoethanol induction of the marrow mesenchymal stem cells (MSCs) to neurons, and to discuss the function of NRSF in neural induction of the MSCs and the mechanism of the differentiation from MSCs to neurons.METHOD:
We used beta-mercaptoethanol, serum-free DMEM, and dimethyl sulfoxide to induce rat MSCs to differentiate to neurons, and then analyzed the changes of the expressions of NRSF gene and NRSF-regulated genes through real-time PCR.RESULTS:
The rat MSCs were successfully induced to differentiate into neuron-like cells. The induced neuron marker, neuron-specific enolase, was positive. Real-time PCR showed that the expression of NRSF gene remarkably declined. The expressions of neurotrophic tyrosine kinase receptor, type 3, synaptosomal-associated protein 25, L1 cell adhesion molecular,neuronal pentraxin receptor in the NRSF-regulated genes also increased at varied extents.CONCLUSIONS:
The differentiation from MSCs to neurons is relevant with the decline of NRSF expression and the increase of the expressions of NRSF-regulated genes. The NRSF may be the key gene during the differentiation from MSCs to neurons.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Células da Medula Óssea
/
Diferenciação Celular
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Células-Tronco Mesenquimais
/
Neurônios
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article