[Screening and identification interaction proteins of connexin 30].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
; 44(9): 758-61, 2009 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-20079101
ABSTRACT
OBJECTIVE:
To explore interaction proteins affect functions of connexin 30 (Cx30) by screening and identification interaction proteins of Cx30.METHODS:
The fusion expression vecto of CX30-C-terminal functional domain-pGEX-4T-2-GST was constructed, and then, fusion protein and GST were purified. They were incubated with the proteins of the foetus brain tissue disruption to pull down interaction proteins. The interaction proteins were separated by SDS-PAGE. Differential straps were cut to enzymolysis to prepare for mass chromatographic analysis, and then to index and screen interaction proteins in NCBInr database. The interaction proteins were identified by immunolocalization.RESULTS:
The four interaction proteins of Cx30 were screened in the foetus brain tissue, as follow, Keratin 16, Camk2b, Tubulin beta-3 and alpha-tubulin. Cx30 was proved to coexist with Keratin 16 and Tubulin beta-3.CONCLUSIONS:
Keratin 16, Camk2b, Tubulin beta-3 and alpha-tubulin are the interaction proteins of Cx30. The interaction proteins affect the assembly, intracellular transport, and channel switch of Cx30.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Conexinas
/
Vetores Genéticos
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article