[Assessment of the Amplicor PCR for the detection of Mycobacterium tuberculosis in smear negative respiratory and non respiratory specimens: a retrospective analysis]. / Análisis retrospectivo del rendimiento de Amplicor-PCR para la detección de Mycobacterium tuberculosis en muestras respiratorias y no respiratorias con baciloscopia negativa.
Rev Chilena Infectol
; 26(6): 495-8, 2009 Dec.
Article
em Es
| MEDLINE
| ID: mdl-20098781
ABSTRACT
BACKGROUND:
Commercial polymerase chain reaction (PCR) kits are widely accepted for analysis of smear positive respiratory specimens, but the sensitivity is variable for smear negative ones.OBJECTIVE:
To assess the PCR method usefulness in smear negative respiratory and non respiratory specimens.METHODS:
We compared the PCR results (AMPLICOR MTB test, Roche) of 235 specimens subjected to culture in Loewenstein-Jensen agar (as the gold standard).RESULTS:
181 (76%) were respiratory and 54 (24%) extra-respiratory specimens. The sensitivity was 88%) and 50%>, respectively, specificity and PPV was 100%> in both cases. NPV was 99.4%> in respiratory specimens and 96.1% in non-respiratory specimens.CONCLUSIONS:
The good performance of this PCR in smear negative respiratory specimens allows the clinician to take decisions based on the result of this exam. In extra-respiratory specimens the contribution is important only when the PCR result is positive.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tuberculose
/
DNA Bacteriano
/
Reação em Cadeia da Polimerase
/
Mycobacterium tuberculosis
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
Es
Ano de publicação:
2009
Tipo de documento:
Article