Expression, purification and preliminary crystallographic analysis of recombinant human DEAD-box polypeptide 5.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 66(Pt 2): 192-4, 2010 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-20124720
ABSTRACT
The DEAD-box RNA helicase DDX5 is involved in many aspects of RNA processing and has been implicated in a number of cellular processes involving alteration of RNA secondary structure. The N-terminal region of DDX5, which contains the conserved domain 1 of the DEAD-box helicases, has been cloned and expressed in Escherichia coli and purified. Here, the crystallization and preliminary diffraction analysis of this region is reported. X-ray diffraction data were processed to a resolution of 2.7 A. The crystals belonged to space group I222, with unit-cell parameters a = 66.18, b = 73.80, c = 104.00 A, alpha = beta = gamma = 90 degrees .
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Helicases DEAD-box
Limite:
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article