Analysis of Cry8Ka5-binding proteins from Anthonomus grandis (Coleoptera: Curculionidae) midgut.
J Invertebr Pathol
; 104(3): 227-30, 2010 Jul.
Article
em En
| MEDLINE
| ID: mdl-20144614
ABSTRACT
Biotech crops expressing Bacillus thuringiensis Cry toxins present a valuable approach for insect control. Cry8Ka5, which is highly toxic to the cotton boll weevil (Anthonomus grandis), was used as a model to study toxin-ligand interactions. Three Cry-binding proteins were detected after toxin overlay assays. Following de novo sequencing, a heat-shock cognate protein and a V-ATPase were identified, whilst a approximately 120 kDa protein remained unknown. Additional Cry8Ka5-binding proteins were visualized by two-dimensional gel electrophoresis ligand blots.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacillus thuringiensis
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Proteínas de Bactérias
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Controle Biológico de Vetores
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Gorgulhos
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Sistema Digestório
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Endotoxinas
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Proteínas Hemolisinas
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Inseticidas
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article