Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.
Biotechnol Bioeng
; 106(2): 319-25, 2010 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-20148400
ABSTRACT
The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion of a human Fc fragment by P. pastoris is not cell-cycle dependent, but rather fluctuates between states of high and low productivity in a stochastic manner.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pichia
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Proteínas Recombinantes
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Técnicas de Cultura de Células
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Perfilação da Expressão Gênica
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Análise Serial de Proteínas
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Modelos Biológicos
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article