Effect of Astragalus membranaceus in rats on peripheral nerve regeneration: in vitro and in vivo studies.
J Trauma
; 68(2): 434-40, 2010 Feb.
Article
em En
| MEDLINE
| ID: mdl-20154555
ABSTRACT
BACKGROUND:
This study provides in vitro and in vivo evaluation of Astragalus membranaceus (AM) on peripheral nerve regeneration.METHODS:
In the in vitro study, we analyzed the effects of AM on cell differentiation and neurite outgrowth by using a PC12 cell model. In the in vivo study, silicone rubber chambers filled with the AM water extract were used to bridge a 10-mm sciatic nerve defect in rats.RESULTS:
We found that the AM water extract caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth and the expression of growth-associated protein 43 from PC12 cells. Animals from the groups treated with the AM for 8 weeks had a relatively more mature structure with larger mean values of myelinated axon number, endoneurial area, and total nerve area when compared with those in the controls receiving the saline only.CONCLUSION:
These results suggest that the silicone rubber tubes present a good framework for the nerve fibers to regenerate across the gap, and the AM extract can be a potential nerve growth-promoting factor, being salutary in aiding the growth of axons in the peripheral nerve.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Astragalus propinquus
/
Fitoterapia
/
Regeneração Nervosa
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article