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Nitric oxide in the hypothalamus-pituitary axis mediates increases in brain glucose retention induced by carotid chemoreceptor stimulation with cyanide in rats.
Cadenas, J L; Montero, S A; Leal, C; Lemus, M; Portilla-de Buen, E; Alvarado, B A; de Alvarez-Buylla, E Roces.
Afiliação
  • Cadenas JL; University Center for Biomedical Research, Universidad de Colima, Ave. 25 de Julio s/n, Colima 28045, Mexico.
Nitric Oxide ; 22(4): 296-303, 2010 May 15.
Article em En | MEDLINE | ID: mdl-20156578
Neuronal nitric oxide synthase (nNOS), which catalyzes the generation of nitric oxide (NO), is expressed by neuron subpopulations in the CNS. Nitric oxide is involved in neurotransmission and central glucose homeostasis. Our prior studies have shown that carotid body receptors participate in brain glucose regulation in vivo, and suggest the presence of a NO tonic mechanism in the solitary tract nucleus (STn). However, the role of NO within STn in glucose control remains unknown. In this study, we explored the potential regulatory role of NO on brain glucose retention induced by carotid body chemoreceptor anoxic stimulation with sodium cyanide (NaCN) which inhibits oxidative metabolism. Intracisternal infusions of nitroxidergic drugs before carotid chemoreceptor stimulation in anesthetized rats, elicited changes in nitrite concentration in plasma and hypothalamus-pituitary (H-P) tissue, as well as in gene expression of neuronal and inducible isoforms (nNOS and iNOS) in H-P tissue. The changes observed in above variables modified brain glucose retention in an opposite direction. When the NO donor, sodium nitroprusside (SNP), was given before carotid stimulation, nitrite concentration in plasma and H-P tissue, and gene expression of nNOS and iNOS in H-P tissue increased, whereas brain glucose retention decreased. In contrast, when the NOS inhibitor, Nomega-nitro-L-arginine methyl ester (L-NAME) was infused immediately before carotid chemoreceptor stimulation, nitrite levels and nNOS expression decreased in plasma and H-P tissue, whereas brain glucose retention increased. Anoxic stimulation by itself induced an increase in the expression of both genes studied. All these results indicate that de novo expression of the nNOS gene in H-P tissue may be critically involved in central glucose changes observed after anoxic carotid chemoreceptor stimulation in conjunction with NO.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cianeto de Sódio / Encéfalo / Células Quimiorreceptoras / Inibidores Enzimáticos / Glucose / Sistema Hipotálamo-Hipofisário / Óxido Nítrico Limite: Animals Idioma: En Ano de publicação: 2010 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cianeto de Sódio / Encéfalo / Células Quimiorreceptoras / Inibidores Enzimáticos / Glucose / Sistema Hipotálamo-Hipofisário / Óxido Nítrico Limite: Animals Idioma: En Ano de publicação: 2010 Tipo de documento: Article