[Effect of lipid-bound apoA-I cysteine mutants upon lipopolysaccharide-induced endotoxemia in mice].
Zhonghua Yi Xue Za Zhi
; 89(44): 3147-50, 2009 Dec 01.
Article
em Zh
| MEDLINE
| ID: mdl-20193280
ABSTRACT
OBJECTIVE:
To determine the anti-inflammatory functions of different cysteine mutants of apolipoprotein A-I recombinant HDLs.METHODS:
The authors reconstituted recombinant HDLs (namely rHDL74, rHDL129, rHDL195 and rHDL228) by mixing wild type or those mutants with dipalmitoyl phosphatidylcholine and examined their in vivo effects upon LPS-induced endotoxemia in mice.RESULTS:
At 24 h post-injection, mice receiving rHDL74 [TNF-alpha (24 +/- 3) pg/ml; IL-1beta (45 +/- 5) pg/ml] had a significant decrease of plasma tumor necrosis factor alpha (TNF-alpha) and interleukin-1beta (IL-1beta) as compared with control mice receiving either saline or rHDLwt [TNF-alpha (135 +/- 12) pg/ml; IL-1beta (82 +/- 8) pg/ml, P < 0.05]. Administration of rHDL74 to mice injected with LPS also led to a protection of lung against acute injury and attenuation of endotoxin-induced clinical symptoms in mice as compared with controls injected with LPS only.CONCLUSION:
Compared with rHDLwt, rHDL74 exhibits higher anti-inflammation capabilities. And it may be a potential clinical candidate for therapy for endotoxin-induced septic shock.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apolipoproteína A-I
/
Endotoxemia
/
Cisteína
/
Lipoproteínas HDL
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article