High-throughput screening in two dimensions: binding intensity and off-rate on a peptide microarray.
Anal Biochem
; 402(1): 93-5, 2010 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-20211590
ABSTRACT
We report a high-throughput two-dimensional microarray-based screen, incorporating both target binding intensity and off-rate, which can be used to analyze thousands of compounds in a single binding assay. Relative binding intensities and time-resolved dissociation are measured for labeled tumor necrosis factor alpha (TNF-alpha) bound to a peptide microarray. The time-resolved dissociation is fitted to a one-component exponential decay model, from which relative dissociation rates are determined for all peptides with binding intensities above background. We show that most peptides with the slowest off-rates on the microarray also have the slowest off-rates when measured by surface plasmon resonance (SPR).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
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Fator de Necrose Tumoral alfa
/
Biblioteca de Peptídeos
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Ressonância de Plasmônio de Superfície
/
Análise Serial de Proteínas
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
/
Screening_studies
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article