Fluorescence quenched quinone methide based activity probes--a cautionary tale.
Org Biomol Chem
; 8(7): 1610-8, 2010 Apr 07.
Article
em En
| MEDLINE
| ID: mdl-20237672
ABSTRACT
A carbamate linked quenching group coupled with a pro-quinone methide reactive core provides an effective tool for studying enzyme function without problems associated with background fluorescence from unreacted probe. However, the relatively slow fragmentation of the carbamate linkage in such a strategy may cause problems of loss of signal or a decoupling of enzyme activity and labelling.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbamatos
/
Arabidopsis
/
Indolquinonas
/
Carboxilesterase
/
Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article