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Comprehensive LC-MS E lipidomic analysis using a shotgun approach and its application to biomarker detection and identification in osteoarthritis patients.
Castro-Perez, Jose M; Kamphorst, Jurre; DeGroot, Jeroen; Lafeber, Floris; Goshawk, Jeff; Yu, Kate; Shockcor, John P; Vreeken, Rob J; Hankemeier, Thomas.
Afiliação
  • Castro-Perez JM; Division of Analytical Biosciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands. josecastroperez@yahoo.com
J Proteome Res ; 9(5): 2377-89, 2010 May 07.
Article em En | MEDLINE | ID: mdl-20355720
A fast and robust method for lipid profiling utilizing liquid chromatography coupled with mass spectrometry has been demonstrated and validated for the analysis of human plasma. This method allowed quantification and identification of lipids in human plasma using parallel alternating low energy and high energy collision spectral acquisition modes. A total of 275 [corrected] lipids were identified and quantified (as relative concentrations) in both positive and negative ion electrospray ionization mode. The method was validated with five nonendogenous lipids, and the linearity (r(2) better than 0.994) and the intraday and interday repeatability (relative standard deviation, 4-6% and 5-8%, respectively) were satisfactory. The developed lipid profiling method was successfully applied for the analysis of plasma from osteoarthritis (OA) patients. The multivariate statistical analysis by partial least-squares-discrimination analysis suggested an altered lipid metabolism associated with osteoarthritis and the release of arachidonic acid from phospholipids.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoartrite / Biomarcadores / Cromatografia Líquida de Alta Pressão / Espectrometria de Massas por Ionização por Electrospray / Lipídeos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Female / Humans Idioma: En Ano de publicação: 2010 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoartrite / Biomarcadores / Cromatografia Líquida de Alta Pressão / Espectrometria de Massas por Ionização por Electrospray / Lipídeos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Female / Humans Idioma: En Ano de publicação: 2010 Tipo de documento: Article