Super-SILAC mix for quantitative proteomics of human tumor tissue.
Nat Methods
; 7(5): 383-5, 2010 May.
Article
em En
| MEDLINE
| ID: mdl-20364148
ABSTRACT
We describe a method to accurately quantify human tumor proteomes by combining a mixture of five stable-isotope labeling by amino acids in cell culture (SILAC)-labeled cell lines with human carcinoma tissue. This generated hundreds of thousands of isotopically labeled peptides in appropriate amounts to serve as internal standards for mass spectrometry-based analysis. By decoupling the labeling from the measurement, this super-SILAC method broadens the scope of SILAC-based proteomics.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Proteômica
/
Marcação por Isótopo
/
Proteínas de Neoplasias
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article