Time- and Spectral-resolved two-photon imaging of healthy bladder mucosa and carcinoma in situ.
Opt Express
; 18(4): 3840-9, 2010 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-20389394
ABSTRACT
Combined non-linear imaging techniques were used to deeply image human ex-vivo fresh biopsies of bladder as well as to discriminate between healthy bladder mucosa and carcinoma in situ. Morphological examination by two-photon excited fluorescence and second-harmonic generation has shown a good agreement with corresponding common routine histology performed on the same samples. Tumor cells appeared slightly different in shape and with a smaller cellular-to-nuclear dimension ratio with respect to corresponding normal cells. Further differences between the two tissue types were found in both spectral emission and fluorescence lifetime distribution by performing temporal- and spectral- resolved analysis of fluorescence. This method may represent a promising tool to be used in a multi-photon endoscope, in a confocal endoscope or in a spectroscopic probe for in-vivo optical diagnosis of bladder cancer.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bexiga Urinária
/
Neoplasias da Bexiga Urinária
/
Aumento da Imagem
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Microscopia de Fluorescência por Excitação Multifotônica
/
Mucosa
Limite:
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article