Establishment and characterization of a new drug surviving cell line Am1010, derived directly from muscle metastases of a human lung adenocarcinoma patient with multi-drug-resistance to cisplatin, taxol, and gefitinib.
Acta Pharmacol Sin
; 31(5): 601-8, 2010 May.
Article
em En
| MEDLINE
| ID: mdl-20418894
ABSTRACT
AIM:
To Characterize a new human lung cancer cell line Am1010, derived from drug-surviving cells (DSCs).METHODS:
The Am1010 cell line was established after 4 cycles of chemotherapy from an arm muscle metastatic tumor of a patient diagnosed with lung adenocarcinoma. The cell line has been remained in continuous culture for more than one year during this study.RESULTS:
The Am1010 cell line demonstrated in vitro multi-drug-resistance to cisplatin, taxol, and gefitinib. The Am1010 cell doubling time without drug treatment was 42.395 h. The IC(50) value of cisplatin was 4.299 micromol/L and >10 micromol/L for the Am1010 and P0318 (a cell line derived from non-DSCs) cells, respectively. The IC(50) value of taxol was 0.067 micromol/L and >1 micromol/L for the Am1010 and P0318 cells, respectively. The IC(50) value of gefitinib was 15.233 micromol/L and >70 micromol/L for Am1010 and P0318 cells, respectively. 11 genes involved in the focal adhesion and cell adhesion pathways were found to be differentially expressed. The cells of Am1010 have a significantly larger chromosome number than most lung cancer cell lines.CONCLUSION:
This novel DSCs derived lung cancer cell line will be a valuable in vitro tool for the investigation of lung cancer drug resistance and metastasis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quinazolinas
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Adenocarcinoma
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Cisplatino
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Paclitaxel
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Resistencia a Medicamentos Antineoplásicos
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Linhagem Celular Tumoral
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Neoplasias Pulmonares
Limite:
Adult
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Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article