A small nucleolar RNA functions in rRNA processing in Caenorhabditis elegans.
Nucleic Acids Res
; 38(17): 5909-18, 2010 Sep.
Article
em En
| MEDLINE
| ID: mdl-20460460
ABSTRACT
CeR-2 RNA is one of the newly identified Caenorhabditis elegans noncoding RNAs (ncRNAs). The characterization of CeR-2 by RNomic studies has failed to classify it into any known ncRNA family. In this study, we examined the spatiotemporal expression patterns of CeR-2 to gain insight into its function. CeR-2 is expressed in most cells from the early embryo to adult stages. The subcellular localization of this RNA is analogous to that of fibrillarin, a major protein of the nucleolus. It was observed that knockdown of C/D small nucleolar ribonucleoproteins (snoRNPs), but not of H/ACA snoRNPs, resulted in the aberrant nucleolar localization of CeR-2 RNA. A mutant worm with a reduced amount of cellular CeR-2 RNA showed changes in its pre-rRNA processing pattern compared with that of the wild-type strain N2. These results suggest that CeR-2 RNA is a C/D snoRNA involved in the processing of rRNAs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Ribossômico
/
Processamento Pós-Transcricional do RNA
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Caenorhabditis elegans
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RNA Nucleolar Pequeno
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article