Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system.
Genesis
; 48(7): 452-6, 2010 Jul.
Article
em En
| MEDLINE
| ID: mdl-20506180
ABSTRACT
The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis-regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large-scale analysis of enhancer elements, site-specifically integrated into the Drosophila genome. This system, which is scalable for either small projects or high-throughput approaches, makes use of the Gateway cloning technology and the PhiC31 site-specific integration system, which allows the insertion of constructs at predetermined genomic locations. Thus, this system allows not only a fast and easy analysis of reporter gene expression in live animals, but also the simultaneous analysis of different regulatory outputs on a cellular resolution by recombining in the same animal distinct enhancer elements fused to different fluorescent proteins.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clonagem Molecular
/
Genes Reporter
/
Integrases
/
Drosophila melanogaster
/
Ensaios de Triagem em Larga Escala
/
Vetores Genéticos
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article