Fully linear reconstruction method for fluorescence yield and lifetime through inverse complex-source formulation: simulation studies.
Opt Lett
; 35(11): 1899-901, 2010 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-20517455
ABSTRACT
In fluorescence imaging, both fluorescence yield and lifetime are of great importance. Traditionally, with the frequency-domain data, two parameters can be directly recovered through a nonlinear formulation. However, the reconstruction accuracy highly depends on initial guesses. To overcome this hurdle, we propose the linear scheme via an inverse complex-source formulation. Using the real and imaginary parts of the frequency-domain data, the proposed method is fully linear; it is not sensitive to initial guesses and is stable with high-level noise. Meanwhile, the algorithm is efficient, and the reconstruction takes one or a few iterations. In addition, the colocalization constraint due to the unique feature of fluorescence imaging is imposed to enhance algorithm performance. The algorithms are tested with simulated data.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Algoritmos
/
Interpretação de Imagem Assistida por Computador
/
Imageamento Tridimensional
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Microscopia de Fluorescência
/
Modelos Biológicos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article