MDM2 SNP 309 human papillomavirus infection in cervical carcinogenesis.
Gynecol Oncol
; 118(3): 258-61, 2010 Sep.
Article
em En
| MEDLINE
| ID: mdl-20538323
ABSTRACT
OBJECTIVE:
To investigate the biological significance of single nucleotide polymorphism (SNP) at murine double-minute 2 homolog (MDM2) promoter 309 in cervical carcinogenesis.METHODS:
SNP at MDM2 promoter 309 (T/G) together with human papillomavirus (HPV) types was examined in a total of 195 cervical smear samples and 8 human cervical squamous carcinoma cell lines using two independent PCR assays and PCR-RFLP techniques.RESULTS:
Forty-one patients with high-grade squamous intraepithelial lesion (HSIL) had higher frequency of high-risk HPV than 102 with low-grade SIL (LSIL) and 52 controls. There was an increased OR (8.88; CI=2.34-33.63; P=0.003) for TG+GG genotype in HSIL cases compared to controls among 68 patients with high-risk HPV. Twenty-one cases with HPV types 16 and/or 18 had significantly higher frequency of the TG+GG genotype and G allele than 47 with other types of high-risk HPV. Seven of 8 cervical carcinoma cell lines also showed TG or GG genotype.CONCLUSION:
MDM2-SNP309 (T/G) and high-risk HPV infection may be closely associated with cervical carcinogenesis in a Japanese population.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
Transformação Celular Viral
/
Neoplasias do Colo do Útero
/
Infecções por Papillomavirus
/
Proteínas Proto-Oncogênicas c-mdm2
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article