The cis/trans isomerization of the double bond of a fatty acid as a strategy for adaptation to changes in ambient temperature in the psychrophilic bacterium, Vibrio sp. strain ABE-1.

ABSTRACT

The major phospholipid, phosphatidylethanolamine (PE), of Vibrio sp. strain ABE-1 contains a unique trans-unsaturated fatty acid, 9-trans-hexadecenoic acid (161(9t], at the sn-2 position of the glycerol moiety. The major molecular species of PE that contain 161(9t) at the sn-2 position have either 9-cis-hexadecenoic acid (161(9c] or hexadecanoic acid (160) at the sn-1 position. The transition temperatures of the liquid-crystal to the gel phase of 161(9c)/161(9t)-PE and 160/161(9t)-PE were -3 degrees C and 38 degrees C, respectively, temperatures that were 31 degrees C and 18 degrees C higher than the corresponding temperatures for 161(9c)/161(9c)-PE and 160/161(9c)-PE. The proportion of 161(9c)/161(9t)-PE and 160/161(9t)-PE increased significantly in cells grown at 20 degrees C over that in cells grown at 5 degrees C. When cells grown at 5 degrees C were incubated at 20 degrees C in the presence of cerulenin, an inhibitor of the synthesis de novo of fatty acids, the level of 161(9t) increased almost in parallel with a concomitant decrease in the level of 161(9c) at the sn-2 position. These results suggest that 161(9c) is converted to 161(9t) by the cis/trans isomerization of the double bond in the fatty acid. This conversion is discussed as a possible strategy for adaptation by bacteria to changes in temperature.