[Ferric iron absorption in deltar p f F xoo, a gene deletion mutant of Xanthomonas oryzae pv. oryzae, assayed using atomic absorption spectrophotometry].
Guang Pu Xue Yu Guang Pu Fen Xi
; 30(4): 1117-9, 2010 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-20545174
ABSTRACT
The ferric iron absorption is one of the most important limiting factors of bacterial growth of Xanthomonas oryzae pv. oryzae. It has been previously speculated that r p f F xoo might be involved in the ferric iron metabolism of the pathogen. In the present study, deltar p f F xoo, a gene deletion mutant, was generated from the wild-type strain PXO99A of Xoo through the homologous recombination, and Fe content was assayed using flame atomic absorption in PXO99A and deltar p f F xoo. The results indicated that the recovery was 99.7% and the relative standard deviation was 1.89 under optimized AAS operating conditions. The increase in Fe absorption in PXO99A and deltar p f F xoo was observed with the increasing time. However, the ferric content of deltar p f F xoo was significantly lower than that of PXO99A (P < 0.05). It is suggested that r p f F xoo is involved in iron metabolism in Xanthomonas oryzae pv. oryzae.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Xanthomonas
/
Deleção de Genes
/
Ferro
Idioma:
Zh
Ano de publicação:
2010
Tipo de documento:
Article