Efficient extraction of nucleolar proteins for interactome analyses.
Proteomics
; 10(16): 3045-50, 2010 Aug.
Article
em En
| MEDLINE
| ID: mdl-20564263
ABSTRACT
The efficient extraction of proteins from purified cellular organelles is critical for in vitro analyses, including identification of protein complex members by affinity purification-based quantitative proteomic approaches. When applied to purified nucleoli, classic nuclear protein extraction methods inefficiently and selectively release only approximately 50% of proteins. Here, we present a method that can extract up to 90% of nucleolar proteins, and apply it in a quantitative interactomic approach to identify nucleolar interaction partners for a mammalian protein phosphatase.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Proteínas Nucleares
/
Nucléolo Celular
/
Proteômica
Limite:
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article