[Mechanisms of foam cell formation in smooth muscle cell derived from bone marrow stem cells].
Nan Fang Yi Ke Da Xue Xue Bao
; 30(6): 1250-2, 2010 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-20584649
ABSTRACT
OBJECTIVE:
To establish a model of smooth muscle cells differentiated from bone mesenchymal stem cells (BMSC-SMCs) in vitro and explore the relationship between scavenger receptors A (SR-A) and caveolin-1.METHODS:
BMSCs were isolated from the femoral bone of SD rats by adherent culture. After treatment of the BMSC-SMCs with 80 mg/L ox-LDL for 72 h, Western blotting was performed to detect the expression of scavenger receptor SR-A, cell cholesterol transport protein ATP-binding cassette transporter Al (ABCA1) and caveolin-1.RESULTS:
BMCS-SMCs became foam cells after treatment with ox-LDL. BMSC-SMC gave rise to more foam cell formation than VSMCs did. Western blotting showed that treatment with 80 mg/L ox-LDL for 72 h resulted in significantly increased expression of SR-A and significantly decreased expressions of ABCA1 and caveolin-1.CONCLUSIONS:
Treatment of BMCS-SMCs with ox-LDL results in cholesterol ester accumulation in the cells to result in foam cells, the mechanism of which involves up-regulation of scavenger receptor SR-A expression and down-regulation of the reverse cholesterol transport protein ABCA1 and caveolin-1 expression.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Caveolina 1
/
Receptores Depuradores Classe A
/
Células-Tronco Mesenquimais
/
Células Espumosas
/
Músculo Liso Vascular
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2010
Tipo de documento:
Article