PCBP1 suppresses the translation of metastasis-associated PRL-3 phosphatase.
Cancer Cell
; 18(1): 52-62, 2010 Jul 13.
Article
em En
| MEDLINE
| ID: mdl-20609352
ABSTRACT
Overexpression of phosphatase of regenerating liver (PRL)-3 is associated with the progression of diverse human cancers. We show that the overexpression of PRL-3 protein is not directly associated with its transcript levels, indicating the existence of an underlying posttranscriptional regulation. The 5' untranslanted region (UTR) of PRL-3 mRNA possesses triple GCCCAG motifs capable of suppressing mRNA translation through interaction with PolyC-RNA-binding protein 1 (PCBP1), which retards PRL-3 mRNA transcript incorporation into polyribosomes. Overexpression of PCBP1 inhibits PRL-3 expression and inactivates AKT, whereas knockdown of PCBP1 causes upregulation of PRL-3 protein levels, activation of AKT, and promotion of tumorigenesis. An inverse correlation between protein levels of PRL-3 and PCBP1 in human primary cancers supports the clinical relevance.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
/
Proteínas Tirosina Fosfatases
/
Ribonucleoproteínas Nucleares Heterogêneas
/
Proteínas de Neoplasias
/
Neoplasias
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article