A single sequence context cannot satisfy all non-AUG initiator codons in yeast.
BMC Microbiol
; 10: 188, 2010 Jul 09.
Article
em En
| MEDLINE
| ID: mdl-20618922
ABSTRACT
BACKGROUND:
Previous studies in Saccharomyces cerevisiae showed that ALA1 (encoding alanyl-tRNA synthetase) and GRS1 (encoding glycyl-tRNA synthetase) respectively use ACG and TTG as their alternative translation initiator codons. To explore if any other non-ATG triplets can act as initiator codons in yeast, ALA1 was used as a reporter for screening.RESULTS:
We show herein that except for AAG and AGG, all triplets that differ from ATG by a single nucleotide were able to serve as initiator codons in ALA1. Among these initiator codons, TTG, CTG, ACG, and ATT had ~50% initiating activities relative to that of ATG, while GTG, ATA, and ATC had ~20% initiating activities relative to that of ATG. Unexpectedly, these non-AUG initiator codons exhibited different preferences toward various sequence contexts. In particular, GTG was one of the most efficient non-ATG initiator codons, while ATA was essentially inactive in the context of GRS1.CONCLUSION:
This finding indicates that a sequence context that is favorable for a given non-ATG initiator codon might not be as favorable for another.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Iniciação Traducional da Cadeia Peptídica
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Saccharomyces cerevisiae
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Códon de Iniciação
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Proteínas de Saccharomyces cerevisiae
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Alanina-tRNA Ligase
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article