The TGFß type II receptor plays a critical role in the endothelial cells during cardiac development.
Dev Dyn
; 239(9): 2435-42, 2010 Sep.
Article
em En
| MEDLINE
| ID: mdl-20652948
ABSTRACT
TGFß signalling is required for normal cardiac development. To investigate which cell types are involved, we used mice carrying a floxed Type II TGFß receptor (Tgfbr2fl) allele and Cre-lox genetics to deplete this receptor in different regions of the heart. The three target tissues and corresponding Cre transgenic lines were atrioventricular myocardium (using cGata6-Cre), ventricular myocardium (using Mlc2v-Cre), and vascular endothelium (using tamoxifen-activated Cdh5(PAC)-CreERT2). Spatio-temporal Cre activity in each case was tracked via lacZ activation from the Rosa26R locus. Atrioventricular-myocardial-specific Tgfbr2 knockout (KO) embryos had short septal leaflets of the tricuspid valve, whereas ventricular myocardial-specific KO embryos mainly exhibited a normal cardiac phenotype. Inactivation of Tgfbr2 in endothelial cells from E11.5 resulted in deficient ventricular septation, accompanied by haemorrhage from cerebral blood vessels. We conclude that TGFß signalling through the Tgfbr2 receptor, in endothelial cells, plays an important role in cardiac development, and is essential for cerebral vascular integrity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator de Crescimento Transformador beta
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Proteínas Serina-Treonina Quinases
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Receptores de Fatores de Crescimento Transformadores beta
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Células Endoteliais
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Coração
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Miocárdio
Limite:
Animals
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Pregnancy
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article