Protection of taurine and granulocyte colony-stimulating factor against excitotoxicity induced by glutamate in primary cortical neurons.
J Biomed Sci
; 17 Suppl 1: S18, 2010 Aug 24.
Article
em En
| MEDLINE
| ID: mdl-20804592
ABSTRACT
BACKGROUND:
Both taurine, an inhibitory neurotransmitter and granulocyte colony-stimulating factor (G-CSF), a growth factor, possess neuroprotective and neurotrophic properties in vitro. However, the mechanisms of their underlying neuroprotective effects are not fully understood.METHODS:
In the present study, we investigated the potential protective benefits of taurine, G-CSF and the combination of taurine and G-CSF against excitotoxicity induced by glutamate in primary cortical neuronal cultures.RESULTS:
25 mM taurine, 25 ng/ml G-CSF and the combination of 25 mM taurine and 25 ng/ml G-CSF showed a protective effect reaching 75%, 75% and 88%, respectively. Furthermore, taurine exerted its protective effect through down-regulation of expression of GRP 78, CHOP, Bim and caspase 12.CONCLUSION:
The results showed that all of these treatments, taurine, G-CSF and the combination of taurine and G-CSF, protected primary cortical neurons against excitotoxicity induced by glutamate. ER stress is suppressed by taurine after glutamate toxicity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Taurina
/
Córtex Cerebral
/
Fator Estimulador de Colônias de Granulócitos
/
Morte Celular
/
Ácido Glutâmico
/
Neurônios
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article