LNA 5'-phosphoramidites for 5'â3'-oligonucleotide synthesis.
Org Biomol Chem
; 8(21): 5012-6, 2010 Nov 07.
Article
em En
| MEDLINE
| ID: mdl-20820663
ABSTRACT
Hereby we report an efficient synthesis of LNA thymine and LNA 5-methylcytosine 5'-phosphoramidites, allowing incorporation of LNA thymine and LNA 5-methylcytosine into oligonucleotides synthesized in the 5'â3' direction. Key steps include regioselective enzymatic benzoylation of the 5'-hydroxy group of unprotected LNA thymine, and subsequent 4,4'-dimethoxytritylation of the 3'-hydroxy group of the O5'-benzoylated LNA thymine nucleoside.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Compostos Organofosforados
/
Timina
/
5-Metilcitosina
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article