Broad detection of diverse H5 and H7 hemagglutinin genes of avian influenza viruses by real-time reverse transcription-PCR using primer and probe sets containing mixed bases.
J Clin Microbiol
; 48(11): 4275-8, 2010 Nov.
Article
em En
| MEDLINE
| ID: mdl-20861344
ABSTRACT
Real-time reverse transcription-PCR (RT-PCR) was developed for broad detection of diverse H5 and H7 genes in Eurasian and American lineages of avian influenza viruses by using primer and probe sets containing mixed bases. Optimal use of the mixed bases enabled us to minimize sequence mismatches and to broaden the gene detection spectrum without decreasing sensitivity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Virologia
/
Glicoproteínas de Hemaglutininação de Vírus da Influenza
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Influenza Aviária
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article