Pharmacological and genetic evidence that cathepsin B is not the physiological activator of rodent prorenin.
Biol Chem
; 391(12): 1469-73, 2010 Dec.
Article
em En
| MEDLINE
| ID: mdl-20868234
ABSTRACT
Renin is the first enzyme in the renin-angiotensin-aldosterone system which is the principal regulator of blood pressure and hydroelectrolyte balance. Previous studies suggest that cathepsin B is the activator of the prorenin zymogen. Here, we show no difference in plasma renin activity, or mean arterial blood pressure between wild-type and cathepsin B knockout mice. To account for potential gene compensation, a potent, selective, reversible cathepsin B inhibitor was developed to determine the role of cathepsin B on prorenin processing in rats. Pharmacological inhibition of cathepsin B in spontaneously hypertensive and double transgenic rats did not result in a reduction in renal mature renin protein levels or plasma renin activity. We conclude that cathepsin B does not play a significant role in this process in rodents.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Catepsina B
/
Renina
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article