Vascular labeling of luminescent gold nanorods enables 3-D microscopy of mouse intestinal capillaries.
ACS Nano
; 4(10): 6278-84, 2010 Oct 26.
Article
em En
| MEDLINE
| ID: mdl-20886812
ABSTRACT
In this study, we aimed to use gold nanorods (Au-NRs) as luminescent substrates for labeling of the mouse intestinal blood vessels for tissue imaging. The labeled intestine was subjected to 3-D confocal microscopy to reveal the intricate morphology of the intestinal capillaries. Using the Au-NR's unique near-infrared excitation and visible fluorescence emission, we observed low noise background compared to the tissue's high autofluorescence from blue laser excitation. We took advantage of this sharp contrast in optical properties to achieve 3-D visualization of the intestinal microstructure and vasculature with capillary-level resolution. This new optical application demonstrates the Au-NR's distinctive properties in vascular labeling and fluorescence microscopy for 3-D illustration of intestinal vasculature.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nanotecnologia
/
Nanopartículas Metálicas
/
Ouro
/
Intestinos
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article