Catechin inhibits adhesion and migration of peripheral blood B cells by blocking CD11b.
Immunopharmacol Immunotoxicol
; 33(2): 391-7, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-20936888
ABSTRACT
CONTEXT Previously, we demonstrated that CD11b is expressed on peripheral blood memory B cells, and it plays an important role in the migration of B cells. And epigallocatechin gallate (EGCG), a bioactive component of green tea, by binding to CD11b, expressed on CD8(+) cytotoxic T cells, inhibited their migratory ability, one possible mechanism of the antiallergic activity of EGCG. OBJECTIVE:
Here, we investigated whether EGCG also affected CD11b expressed on B cells, similar to cytotoxic T cells. MATERIALS ANDMETHODS:
Isolated peripheral blood CD19(+) B cells were treated with EGCG and the change in the expression of CD11b was analyzed using flow cytometry. The effects of EGCG on the ability of B cells to adhere to and to transmigrate through the endothelial cell layer were evaluated using the transwell assay.RESULTS:
EGCG significantly suppressed the apparent expression of CD11b on B cells, in the flow-cytometric analysis, and this apparent suppression was speculated to be dependent on the competitive binding of EGCG to CD11b. EGCG also significantly suppressed CD11b-mediated migration and adhesion of B cells to endothelial cells. DISCUSSION ANDCONCLUSION:
EGCG has a strong suppressive activity on the adhesive and migratory abilities of peripheral blood B cells. This suppressive activity was mediated by the binding of EGCG to CD11b on B cells, and the consequent suppression of B-cell extravasation to the extravascular space. Because B cell plays an important role in the humoral immunity, EGCG could be a promising drug for the prevention and/or treatment of allergic and/or autoimmune diseases.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Catequina
/
Movimento Celular
/
Subpopulações de Linfócitos B
/
Antígeno CD11b
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article