In situ monitoring of antibiotic susceptibility of bacterial biofilms in a microfluidic device.
Lab Chip
; 10(23): 3296-9, 2010 Dec 07.
Article
em En
| MEDLINE
| ID: mdl-20938507
ABSTRACT
Antibiotic resistance of biofilms is a growing public health concern due to overuse and improper use of antibiotics. Thus, determining an effective minimal concentration of antibiotics to eradicate bacterial biofilms is crucial. Here we present a simple, novel one-pot assay for the analysis of antibiotic susceptibility of bacterial biofilms using a microfluidics system where continuous concentration gradients of antibiotics are generated. The results of minimal biofilm eradication concentration (MBEC) clearly confirm that the concentration required to eradicate biofilm-grown Pseudomonas aeruginosa is higher than the minimal inhibitory concentration (MIC) that has been widely used to determine the lowest concentration of antibiotics against planktonically grown bacteria.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
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Testes de Sensibilidade Microbiana
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Biofilmes
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Microfluídica
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Procedimentos Analíticos em Microchip
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Dispositivos Lab-On-A-Chip
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Antibacterianos
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article