Characterization of a yeast culture extract compound stimulating the growth of an anaerobic cellulolytic consortium.

ABSTRACT

In this work the compound obtained from yeast culture extract (YCE) that stimulated the activity of an anaerobic cellulolytic consortium (ACC) was characterized. YCE were obtained at different pH (4, 7 and 10) and ultra-filtered 300 and 30 kDa membranes (UYE). The 30 kDa UYE was heated to 60 °C, 90 °C and 120 °C and gel filtered (GYF). Mid infrared spectroscopy, protein and carbohydrate analysis of GYF were conducted. Results showed that YCE, UYE and GYF significantly stimulated (p < 0.05) the biomass production, acetate concentration and carboxymethyl cellulase activity of the ACC, in relation to the control. The GYF had an estimated molecular mass of 4 kDa. Mid-infrared and biochemical analysis of GYF suggested that the active compound is a peptide.