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Potential action of copper surfaces on meticillin-resistant Staphylococcus aureus.
Weaver, L; Noyce, J O; Michels, H T; Keevil, C W.
Afiliação
  • Weaver L; Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Southampton, UK. louise.weaver@esr.cri.govt.nz
J Appl Microbiol ; 109(6): 2200-5, 2010 Dec.
Article em En | MEDLINE | ID: mdl-21040269
AIMS: Studies to date have shown rapid killing of bacterial cells when exposed to copper surfaces. The mechanistic action of copper on bacterial cells is so far unknown. METHODS AND RESULTS: To investigate potential mechanisms involved, meticillin-resistant Staphylococcus aureus (MRSA) cells (10(7) CFU) were inoculated onto coupons of copper or stainless steel and stained with either the viability fluorophore 5-cyano-2,3-ditolyl tetrazolium (CTC), to detect respiration, or BacLight™ (SYTO9/propidium iodide), to determine cell wall integrity. Coupons were then observed in-situ using epifluorescence microscopy. In addition, DNA from cells inoculated onto either copper or stainless steel surfaces was isolated and analysed by agarose gel electrophoresis. An effect on cellular respiration with CTC reduction was evident but no effect on cell membrane integrity (BacLight™) was observed. Results from the DNA isolation indicated a copper-induced detrimental effect on MRSA genomic material as no bands were observed after exposure to copper surface. CONCLUSIONS: The results indicate that exposure to copper surfaces rapidly kills MRSA by compromising cellular respiration and damaging DNA, with little effect on cell membrane integrity. SIGNIFICANCE AND IMPACT OF THE STUDY: This research provides a mechanistic explanation in support of previous suggestions that although copper surfaces do not affect membrane integrity of cells, there is still a rapid antimicrobial effect.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cobre / Staphylococcus aureus Resistente à Meticilina Idioma: En Ano de publicação: 2010 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cobre / Staphylococcus aureus Resistente à Meticilina Idioma: En Ano de publicação: 2010 Tipo de documento: Article