Kaposi's sarcoma-associated herpesvirus-encoded LANA positively affects on ubiquitylation of p53.
Biochem Biophys Res Commun
; 403(2): 194-7, 2010 Dec 10.
Article
em En
| MEDLINE
| ID: mdl-21056547
ABSTRACT
We established a series of stable transfectants expressing wild-type and three mutant LANA; amino terminus, carboxyl terminus and amino terminus plus DNA binding domain, as a new strategy to assess systematically the interactions and binding domains with cellular proteins. Using the system, we reported that LANA specifically bound to p53 via DNA binding domain. As for LANA function in the regulation of p53 through the interaction, we showed that polyubiquitylation of p53 in the presence of LANA was obviously increased. LANA also associated with Cullin 5 and Rbx1, active subunit of E3 ubiquitin ligase complex. Taken together, the present study suggests that LANA induce enhancement of p53 ubiquitylation and degradation into proteasome, consequently contributing to latent persistence.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Nucleares
/
Proteína Supressora de Tumor p53
/
Ubiquitinação
/
Antígenos Virais
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article