Expression of heterobispecific antibodies by genes transfected into producer hybridoma cells.
Gene
; 87(2): 213-8, 1990 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-2110103
ABSTRACT
We report the expression of heterobispecific antibodies (Ab) by transferred genes. The kappa and gamma 1 genes of a mouse anti-idiotypic Ab (IgG1) were transfected into a mouse hybridoma cell line secreting Ab (IgG1), directed against an isoenzyme of human creatine kinase. Stable cell lines secreting the parental Ab derived from the introduced genes and a mixture of hybrid Ab were established. The transfected Ab specificity was expressed at similar levels as in a nonproducer background (50 ng-1 microgram/ml), heterobispecific Ab was expressed in microgram quantities (1-4 micrograms/ml) in all cell lines examined. As shown by isoelectric focusing analysis, hybrid Ab (heterobifunctional and other species) are expressed to a similar extent in the transfected cell lines as the Ab in the parental Ab-producing cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes de Imunoglobulinas
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Transfecção
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Cadeias gama de Imunoglobulina
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Cadeias Pesadas de Imunoglobulinas
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Cadeias kappa de Imunoglobulina
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Especificidade de Anticorpos
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article