Involvement of protein synthesis in recovery from refractory period of electrical depolarization induced by osmotic stimulation in Chara corallina.
J Plant Res
; 124(5): 639-44, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-21104191
ABSTRACT
Upon addition of sorbitol to the external medium of an internodal cell of Chara corallina, a transient depolarization is induced at its nodal end (Shimmen in Plant Cell Physiol 441215-1224, 2003). In the present study, refractory period was found to be very long, 2-4 h. Recovery from refractoriness was completely inhibited by inhibitors of eukaryote-type protein synthesis, cycloheximide or anisomysin, but not by inhibitors of prokaryote-type protein synthesis. This suggested that proteinous factor(s) responsible for generation of the depolarization is lost or inactivated upon depolarization and synthesized during the resting state. Low temperature, which is supposed to inhibit protein synthesis, also inhibited recovery from refractoriness. When unstimulated internodal cells were incubated in the medium containing an inhibitor of eukaryote-type protein synthesis, generation of the depolarization was almost completely inhibited. This result suggested that the factor is slowly turning over even in the absence of osmotic stimulation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Período Refratário Eletrofisiológico
/
Sorbitol
/
Biossíntese de Proteínas
/
Chara
/
Potenciais da Membrana
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article