Critical interaction domains between bloom syndrome protein and RAD51.
Protein J
; 30(1): 1-8, 2011 Jan.
Article
em En
| MEDLINE
| ID: mdl-21113733
ABSTRACT
The American Cancer Society's 2009 statistics estimate that 1 out of every 4 deaths is cancer related. Genomic instability is a common feature of cancerous states, and an increase in genomic instability is the diagnostic feature of Bloom Syndrome. Bloom Syndrome, a rare disorder characterized by a predisposition to cancer, is caused by mutations of the BLM gene. This study focuses on the partnerships of BLM protein to RAD51, a Homologous Recombination repair protein essential for survival. A systematic set of BLM deletion fragments were generated to refine the protein binding domains of BLM to RAD51 and determine interacting regions of BLM and ssDNA. Results show that RAD51 and ssDNA interact in overlapping regions; BLM100â214 and BLM1317â1367. The overlapping nature of these regions suggests a preferential binding for one partner that could function to regulate homologous recombination and therefore helps to clarify the role of BLM in maintaining genomic stability.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Instabilidade Genômica
/
Rad51 Recombinase
/
RecQ Helicases
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Domínios e Motivos de Interação entre Proteínas
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article