Expression, purification, crystallization and initial X-ray diffraction analysis of thiol peroxidase from Yersinia pseudotuberculosis.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 66(Pt 12): 1606-9, 2010 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-21139206
ABSTRACT
Thiol peroxidase is an atypical 2-Cys peroxiredoxin that reduces alkyl hydroperoxides. Wild-type and C61S mutant protein have been recombinantly expressed in Escherichia coli and purified using nickel-affinity chromatography. Initial crystallization trials yielded three crystal forms in three different space groups (P2(1), P6(4) and P2(1)2(1)2(1)) both in the presence and the absence of DTT.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peroxidases
/
Difração de Raios X
/
Yersinia pseudotuberculosis
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article