Cross-species chemogenomic profiling reveals evolutionarily conserved drug mode of action.
Mol Syst Biol
; 6: 451, 2010 Dec 21.
Article
em En
| MEDLINE
| ID: mdl-21179023
ABSTRACT
We present a cross-species chemogenomic screening platform using libraries of haploid deletion mutants from two yeast species, Saccharomyces cerevisiae and Schizosaccharomyces pombe. We screened a set of compounds of known and unknown mode of action (MoA) and derived quantitative drug scores (or D-scores), identifying mutants that are either sensitive or resistant to particular compounds. We found that compound-functional module relationships are more conserved than individual compound-gene interactions between these two species. Furthermore, we observed that combining data from both species allows for more accurate prediction of MoA. Finally, using this platform, we identified a novel small molecule that acts as a DNA damaging agent and demonstrate that its MoA is conserved in human cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Schizosaccharomyces
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Testes de Sensibilidade Microbiana
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Regulação Fúngica da Expressão Gênica
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Farmacorresistência Fúngica
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Antifúngicos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article