Whole gene amplification and protein separation from a few cells.
Anal Biochem
; 411(1): 64-70, 2011 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-21185803
ABSTRACT
Despite the growing interest to explore untapped microbial gene and protein diversity, no single platform has been able to acquire both gene and protein information from just a few cells. We present a microfluidic system that simultaneously performs on-chip capillary electrophoresis for protein analysis and whole genome amplification (WGA), and we demonstrate this by doing both for the same cohort of cyanobacterial cells. This technology opens avenues for studying protein profiles of precious environmental microbial samples and simultaneously accessing genomic information based on WGA.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Técnicas de Amplificação de Ácido Nucleico
/
Synechococcus
/
Genes Bacterianos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article