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Recovering lost magnetization: polarization enhancement in biomolecular NMR.
Favier, Adrien; Brutscher, Bernhard.
Afiliação
  • Favier A; IBS, Institut de Biologie Structurale Jean-Pierre Ebel, 41 rue Jules Horowitz, 38027 Grenoble, France.
J Biomol NMR ; 49(1): 9-15, 2011 Jan.
Article em En | MEDLINE | ID: mdl-21190063
Experimental sensitivity remains a major drawback for the application of NMR spectroscopy to fragile and low concentrated biomolecular samples. Here we describe an efficient polarization enhancement mechanism in longitudinal-relaxation enhanced fast-pulsing triple-resonance experiments. By recovering undetectable (1)H polarization originating from longitudinal relaxation during the pulse sequence, the steady-state (15)N polarization becomes enhanced by up to a factor of ~5 with respect to thermal equilibrium yielding significant sensitivity improvements compared to conventional schemes. The benefits of BEST-TROSY experiments at high magnetic field strength are illustrated for various protein applications, but they will be equally useful for other protonated macromolecular systems.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas / Ressonância Magnética Nuclear Biomolecular / Magnetismo Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2011 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas / Ressonância Magnética Nuclear Biomolecular / Magnetismo Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2011 Tipo de documento: Article