Synthesis of modified siRNA bearing C-5 polyamine-substituted pyrimidine nucleoside in their 3'-overhang regions and its RNAi activity.
Bioorg Med Chem Lett
; 21(2): 715-7, 2011 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-21190853
ABSTRACT
Short interfering RNA (siRNA) induces specific gene silencing by the RNA interference (RNAi) pathway. Nucleosides in the 3'-overhang regions of siRNAs were replaced with 5-bis(aminoethyl)aminoethylcarbamoylmethyl-2'-deoxyuridine or thymidine. siRNA bearing modified nucleoside was more active in silencing the gene expression of hepatocyte nuclear factor 4α (HNF4α) compared with siRNA bearing thymidine.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poliaminas
/
Nucleosídeos de Pirimidina
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Fator 4 Nuclear de Hepatócito
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article