[Effect of tamoxifen on proliferation of cultured breast cancer and cervical carcinoma cell lines].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
; 19(2): 189-92, 2003 May.
Article
em Zh
| MEDLINE
| ID: mdl-21207675
ABSTRACT
AIM:
To investigate the effects of tamoxifen on proliferation of human breast cancer Bcap-37 cells and cervical carcinoma HeLa cells and to explore it's possible mechanism.METHODS:
The techniques of cell culture, growth curves, flow cytometry and laser scanning confocal microscope were used.RESULTS:
Tamoxifen (10(-6) mol/L) shifted the growth curve of Bcap-37 cells downward, and shifted the growth curve of HeLa cells upward. Tamoxifen (10(-8) - 10(-6) mol/L) inhibited the proliferation of Bcap-37 cells in a dose-dependent manner, but stimulated the proliferation of HeLa cells in a dose-dependent manner. Bcap-37 cells appeared apoptosis when treated with tamoxifen (10(-6) mol/L), and the same dose stimulated the proliferation of HeLa cells at GI/S phases. The apoptotic rate of Bcap-37 cells was 97.5%. It blocked G1 phase of HeLa cells from 55.5% to 32.8%, and increased the S phase from 29.0% to 49.4%. Tamoxifen (10(-6) mol/L) also increased the releasing of calcium in Bcap-37 and HeLa cells.CONCLUSION:
Tamoxifen can significantly influence the proliferation of breast cancer and cervical carcinoma cells possibly by affecting cell cycle and stimulating the releasing of Ca2+ in the cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tamoxifeno
/
Neoplasias da Mama
/
Neoplasias do Colo do Útero
/
Proliferação de Células
Limite:
Female
/
Humans
Idioma:
Zh
Ano de publicação:
2003
Tipo de documento:
Article