Impact of systemic immuno-suppression after solid organ transplantation on allergen-specific responses.
Allergy
; 66(2): 271-8, 2011 Feb.
Article
em En
| MEDLINE
| ID: mdl-21208218
ABSTRACT
INTRODUCTION:
The immunosuppressive therapy in solid organ transplantation targets mainly the T- and B-cell-mediated immune response. However, there is evidence that it neither suppresses sensitization nor clinical manifestation of allergic diseases in organ-transplanted patients.OBJECTIVE:
This study addresses the question whether allergen-specific responses are altered by systemic immunosuppression via negative effects on the T-regulatory cell compartment and a more pronounced suppression on Th1-type T-cell responses. MATERIAL ANDMETHODS:
Peripheral blood mononuclear cells from 65 solid organ-transplanted (kidney, liver, lung) children, adolescents, and young adults and 18 healthy, matched controls were included, and their clinical and sensitization status assessed. Allergen-specific proliferation, intracellular cytokine production, frequency of forkhead box P3 (FOXP3)+ CD3+ CD4+ CD25(high) cells, mRNA expression of IL-10, transforming growth factor (TGF)-ß and FOXP3 (real-time RT-PCR) of peripheral blood mononuclear cells or bronchoalveolar lavage fluid (BAL)-derived cells, and the inhibitory capacity of T-reg cells were investigated.RESULTS:
Immunosuppression led to a significantly altered regulatory marker profile expressed by enhanced TGF-ß mRNA production and a reduced frequency of FOXP3+ CD4+ CD3+ cells in solid organ transplanted individuals. FOXP3 expression in BAL cells of lung-transplanted patients was significantly decreased. Allergen-specific proliferation was not significantly altered despite long-term immunosuppression. However, suppression of allergen-specific responses via the T-regulatory cell fraction was deficient in immunosuppressed individuals.CONCLUSION:
The results suggest an insufficient control of allergen-specific responses via the Treg-cell compartment under systemic immunosuppression.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alérgenos
/
Transplante de Órgãos
/
Terapia de Imunossupressão
/
Linfócitos T Reguladores
/
Especificidade do Receptor de Antígeno de Linfócitos T
/
Hipersensibilidade
Tipo de estudo:
Observational_studies
Limite:
Adolescent
/
Adult
/
Child
/
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article